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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Transfusion. 2018 Oct 12;58(12):2978–2991. doi: 10.1111/trf.14936

Figure 4 – Methylation of isoaspartate and deamidated asparagine residues in human hemoglobin alpha and beta,

Figure 4 –

as mapped on their sequence (A), highlighted in the deoxyhemoglobin structure (based on pdb: 1a3n – B) and shown in a representative MS/MS spectrum (methylation of D22 – C). A top and side view of deoxyhemoglobin, along with the N-term of band 3 that has been shown to interact with the deoxyhemoglobin tetramer is shown in D. D-methyl and N-deamidated residues are highlighted in red and green, respectively.