Fig. 2.

Effect of microinjection of A-779 into the PVN on superoxide and gp91^phox in the PVN of rats with salt-induced hypertension. Immunofluorescence for the NAD(P)H oxidase subunit gp91^phox and dihydroethidium (DHE) was performed on PVN tissue sections. A Immunofluorescence for gp91^phox (bright red) and superoxide (bright red, labeled by DHE) in the PVN in different groups. Nuclei were labeled with DAPI (blue). B, C Histograms showing the effects of microinjection of A-779 into the PVN on the number of gp91^phox-positive cells (B) and superoxide-positive cells (C) in the PVN of different groups. Values are presented as mean ± SEM; *P < 0.05 versus NS groups (NS + PVN A-779 or NS + PVN vehicle); ^†P < 0.05 HS + PVN A-779 versus HS + PVN vehicle.