Table 2.
Gene | Sequence | |
---|---|---|
GAPDH | Forward primer | 5′CTGGAAGATGGTGATGG3′ |
Reverse primer | 5′GATTTGGTCGTATTGGGCG3′ | |
Aggrecan | Forward primer | 5′TCGCAAGTCCCTTCCACATC3′ |
Reverse primer | 5′TCAAGGCGTCCTGAAGTGTC3′ | |
Collagen type II | Forward primer | 5′GCTGGTGCACAAGGTCCTAT3′ |
Reverse primer | 5′AGGGCCAGAAGTACCCTGAT3′ | |
Sox9 | Forward primer | 5′CCAGCAAGAACAAGCCACAC3′ |
Reverse primer | 5′CTTGCCCAGAGTCTTGCTGA3′ |
RT-PCR: reverse transcription polymerase chain reaction; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.