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. 2019 Jan 15;11(1):509–518.

Figure 3.

Figure 3

HSP90 inhibitor 17-DMAG alleviated the loss of elastic fibers integrity and reduction of SMCs contraction. A. Representative image of hematoxylin-eosin (HE) and victoria blue (VB) staining in aortic walls. Serious degeneration and fragmentation of elastic fibers in BAPN group, however the breakage of elastic fibers was significantly alleviated in 17-DMAG group. B and C. The mRNA and protein expression of SM22, OPN and HSP90 in aortic walls tissues. Real-time PCR and western blot assays were performed on aortic walls tissues from control, BAPN and 17-DMAG groups (n=6 for each group) respectively. Treatment of 17-DMAG could significantly attenuate the expression change of SM22, OPN and HSP90. **P<0.01 vs control group, ##P<0.01 vs BAPN group. D. Representative image of immunofluorescence staining with HSP90 antibody (red) and α-SMA antibody (green). HSP90 was highly expressed in aortic walls from BAPN-treated mice, and mainly located in smooth muscle cells. DAPI (blue) was used for nuclei staining.