Figure 1.

Resveratrol increased survival area of random skin flaps, decreased tissue edema and improved angiogenesis in the flaps. After the modified “McFarlane flap” model was established, the cutaneous appearance and edema of skin flaps were observed and recorded, and neovascularization was assessed by the number of micro vessels in dermis layer. A. Digital photographs of flap survival in the Control and Rev groups were taken on day3 and day7. B. The percentages of survival area in two groups were quantified and analyzed. C. Digital photographs of the inner side of the skin flap were taken from the Control and Rev groups to show the degree of tissue edema. D. Histogram of percentage of tissue water content in each group. E. Laser doppler blood flow (LDBF) imaging of flaps in each group to show the blood supply in the tissue. F. The percentages of area with blood flow in flaps were quantified and analyzed. G. H&E staining to show vessels in area II of flap in the Control group and the Rev group (original magnification × 200; scan bar, 50 μm). H. The MVDs in each group were quantified and analyzed. I. IHC for CD34 to present vessels in area II in each group. J. The CD34-positive vessel densities in the two groups were quantified and analyzed. Significance: *P < 0.05 and **P < 0.01 vs. the Control group. Data were expressed as means ± SEM, n = 6 per group.