Figure 4.

Resveratrol attenuated oxidative stress in ischemic area of the flaps. The oxidative stress in flap area II was assessed by IHC for SOD1, Western blotting for SOD1, eNOS and HO1, and assays for SOD, GSH and MDA activities on day 7. A. IHC for SOD1 expression in area II of random skin flaps in the Control and Rev groups (original magnification, × 200; scan bar, 100 μm). B. Optical density values of SOD1 expression were quantified and analyzed in each group. C-E. Western blotting for the expression of SOD1, eNOS and HO1 in Area II flaps in the Control group and the Rev group. The gels were run under the same experimental conditions, and cropped blots are used here. The Original images are available in Figure S1C. F-H. Optical density values of the expression of SOD1, eNOS and HO1 were quantified and analyzed in each group. I-K. Values of SOD activities, GSH levels and MDA content in flap area II of each group via xanthine oxidase method, the modified 5,5’-dithiobis method and the modified TBA test were quantified and analyzed, respectively. Significance: *P < 0.05 and **P < 0.01 vs. the Control group. Data were expressed as means ± SEM, n = 6 per group.