Fig. 3.

PLA2 activities of the periplasmic and cytoplasmic fractions of Rs-A2. Rs-A2 was grown photoheterotrophically in Sis minimal medium. Periplasmic and cytoplasmic fractions were prepared from cells harvested at the exponential growth phase (OD₆₆₀ of 2.3). The PLA activity in each fraction was determined with membrane lipids from the photoheterotrophically grown WT cells and expressed as the percentage of the total activity, which amounted to approximately 111.5 U mg⁻¹ protein. One enzyme unit was defined as 1 µmol of FFAs released per min from PLs under the assay conditions examined. PLA2 activities are shown with error bars from three independent experiments