Fig. 7.

FFA productivity of Rs-HCA2 grown at high cell density in a two-phase system with dodecane. a Rs-HCA2 was grown photoheterotrophically in SisH-D medium. Dodecane was added to the culture at concentrations of 15%, 30%, and 50% (v/v). The culture broth and dodecane layer were harvested at the exponential growth phase (OD₆₆₀ of 5.3 for Rs-HCA2 in the absence of dodecane; OD₆₆₀ of 16.0, 16.0 and 3.2 for Rs-HCA2 in the presence of 15%, 30% and 50% dodecane, respectively). FFAs in the culture supernatants (open bar) and in the dodecane layer (closed bar) were measured, and the FFA productivity is illustrated with error bars from three independent experiments. b Rs-HCA2 was cultured in SisH-D medium in the presence of varying levels of dodecane (0, 15, 30 and 50%) under photoheterotrophic conditions; data shown are for one of three representative experiments