Table 1.
Strains and plasmids used in this study
| Strains/plasmids | Relevant characteristics | References/description |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5αphe | phe::Tn10dCm of DH5α | [32] |
| HB101 | F− mcrB mrr hsdS20(r−B m−B) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ− | [33] |
| S17-1 | C600::RP4 2-(Tc::Mu)(Km::Tn7) thi pro hsdR hsdM+ recA | [34] |
| R. sphaeroides | ||
| KD131 | Type strain | [25] |
| Rs-nA2 | R. sphaeroides containing pIND-PLA2 | This study |
| Rs-A2 | R. sphaeroides containing pINDA′-PLA2 | This study |
| Rs-nA2-phoA | R. sphaeroides containing pIND-PLA2-phoA | This study |
| Rs-A2-phoA | R. sphaeroides containing pINDA′-PLA2-phoA | This study |
| Rs-C | R. sphaeroides containing pLVplsC | This study |
| Rs-DC | R. sphaeroides containing pRKfabD and pLVplsC | This study |
| Rs-AccC | R. sphaeroides containing pRKaccABCD and pLVplsC | This study |
| Rs-HC | R. sphaeroides containing pRKfabH and pLVplsC | This study |
| Rs-AccCA2 | R. sphaeroides containing pRKaccABCD, pLVplsC and pINDA′-PLA2 | This study |
| Rs-CA2 | R. sphaeroides containing pLVplsC and pINDA′-PLA2 | This study |
| Rs-DCA2 | R. sphaeroides containing pRKfabD, pLVplsC and pINDA′-PLA2 | This study |
| Rs-HCA2 | R. sphaeroides containing pRKfabH, pLVplsC and pINDA′-PLA2 | This study |
| Rs-HXYCA2 | R. sphaeroides containing pRKfabH, pBBRplsX, pLVplsCY and pINDA′-PLA2 | This study |
| Rs-tol | A tolC mutant; Tpr | This study |
| Rs-A2tol | A tolC mutant containing pINDA′-PLA2; Tpr | This study |
| Plasmids | ||
| pBBR1MCS-2 | ori pBBR1 lacZa; Kmr | [35] |
| pBBRplsX | pBBR + 1.3-kb HindIII–XbaI fragment containing PlsX; Kmr | This study |
| pBS(−) | ori f1 lacZ′; Apr | Stratagene |
| pBSaccA | pBS + 1.1-kb XbaI–PstI fragment containing accA; Apr | This study |
| pBSaccD | pBS + 1.3-kb XbaI–PstI fragment containing accD; Apr | This study |
| pBSfabD | pBS + 1.2-kb XbaI–PstI fragment containing fabD; Apr | This study |
| pMD20 | ori f1 lacZ′; Apr | Takara |
| pMDaccBC | pMD + 2.9-kb HindIII–XbaI fragment containing accBC; Apr | This study |
| pIND4 | ori ColE1 IPTG-inducible promoter; Kmr | [29] |
| pIND4S | ori ColE1 IPTG-inducible promoter; Sm/Spr | This study |
| pIND-PLA2 | pIND4S + 387-bp NcoI–HindIII fragment containing PLA2; Sm/Spr | This study |
| pINDA′-PLA2 | pIND4S + 456-bp NcoI–HindIII fragment containing cycA′-PLA2; Sm/Spr | This study |
| pINDA′-PLA1 | pIND4S + 1.9-kb NcoI–HindIII fragment containing cycA′-PLA1; Sm/Spr | This study |
| pIND-PLA2-phoA | pIND4S + 1.9-kb NcoI–HindIII fragment containing PLA2-phoA; Sm/Spr | This study |
| pINDA′-PLA2-phoA | pIND4S + 2.0-kb NcoI–HindIII fragment containing cycA′-PLA2-phoA; Sm/Spr | This study |
| pLV106 | IncQ pUI511 containing the polylinker of EcoRI, SmaI, KpnI, and XbaI restriction sites inserted at PstI site | [36] |
| pLV-Tp | pLV106 + 1.6-kb HindIII fragment containing Tpr gene | This study |
| pLVplsC | pLV-Tp + 1.0-kb XbaI–KpnI fragment containing plsC; Tpr | This study |
| pLVplsCY | pLV-Tp + 1.0-kb XbaI–KpnI fragment containing plsC and 0.8-kb KpnI-EcoRI fragment containing plsY; Tpr | This study |
| pRK2013 | ori ColE1; Kmr | [37] |
| pRK415 | ori IncP-1 Mob RP4 lacZa; Tcr | [38] |
| pRKfabH | pRK415 + 1.8-kb KpnI–XbaI fragment containing fabH; Tcr | This study |
| pRKfabD | pRK415 + 1.2-kb XbaI–KpnI fragment containing fabD; Tcr | This study |
| pRKaccABCD | pRK415 + 5.3-kb XbaI–KpnI fragment containing accABCD; Tcr | This study |
| pSUP202 | ori p15A Mob RP4; Tcr Cmr Apr | [39] |
| pSUPTolC | pSUP202 + 2.6-kb DNA of tolC::Tp; tolC interrupted by 1.6-kb Tpr DNA; Tpr Tcr | This study |