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Additional file 3: Movie S2. Playback at 5 frames per second, each frame represents 1 z-stack capture per minute. This movie shows “normal” wild type peristaltic movements of a 7 dpf zebrafish, highlighting the intestinal region starting at the intestinal bulb-upper intestine junction. Fluorescent microspheres can be seen moving posteriorly down the digestive tract. Post processing was done to reduce size, change orientation of images, and speed playback up. (MP4 23290 kb)