Table 1.
Genotype of all 19 pups born following TALEN injections
| # | Pup ID | Genotype by Sanger sequencing | ||
|---|---|---|---|---|
| H2Afb3 | Gm14920 | H2Afb2 | ||
| 1 | ♀ L74-1 | XX (wt) | XX (wt) | XX (wt) |
| 2 | ♀ L74-2 | XX (wt) | XX (wt) | XX (wt) |
| 3 | ♀ L74-3 | XX (wt) | XX (wt) | XX (wt) |
| 4 | ♂ L74-4 | XY (wt) | XY (wt) | XY (wt) |
| 5 | ♂ L74-5* | X ∆5 + 286 Y | X ∆15 Y | X ∆17 Y |
| 6 | ♀ L79-1 | X ∆10 X ∆10 |
X
∆12
X
∆12
Chimera of b/∆/c |
X
∆15
X
∆15
Chimera of c/∆/b |
| 7 | ♀ L89-1* | X ∆22 X ∆160 |
X
∆5
X
∆17
Chimera c/∆/b) |
X ∆5 X ∆5 |
| 8 | ♂ L89-2 | X ∆65 Y | X C/T, A/G Y | X∆42 Y Chimera b/∆/c) |
| 9 | ♂ L89-3 |
X
∆26
Y
Chimera c/∆/a) |
X
∆10
Y
Chimera b/∆/c) |
X
∆10
Y
Chimera b/∆/c) |
| 10 | ♀ L90-1 | XX (wt) | XX (wt) | XX (wt) |
| 11 | ♀ L90-2 | XX (wt) | XX (wt) | XX (wt) |
| 12 | ♀ L90-3* | X ∆28 X ∆5 | X ∆10 X ∆10 | X ∆64 X ∆64 |
| 13 | ♀ L90-4 | X ∆15 X ∆10 | No successful PCR amplification | No successful PCR amplification. |
| 14 | ♀ L90-5 | XX (wt) | XX (wt) | XX (wt) |
| 15 | ♀ L90-6 |
X
∆17
X
∆17
Chimera c/∆/a) |
X
∆5
X
∆5
Chimera b/∆/c) |
X
∆5
X
∆5
Chimera c/b/∆/c) |
| 16 | ♀ L90-7 | XX (wt) | XX (wt) | XX (wt) |
| 17 | ♂ L90-8 | X ∆16 Y |
X
∆17
Y
Chimera c/∆/c/b) |
No successful PCR amplification. |
| 18 | ♂ L90-9 | XY (wt) | XY (wt) | XY (wt) |
| 19 | ♂ L90-10 | XY (wt) | XY (wt) | XY (wt) |
wt, wild type offspring; ∆n, deletion of n nucleotides; +n, insertion of n nucleotides. *Denotes mice that were used as founders to establish H2A.B.3 KO colonies. The nine Talen induced mutations are italicized