Fig. 6.

Effects of Apelin 13 were through AMPK and GSK-3β. a Apelin 13 increased the phosphorylation levels of AMPK and GSK-3β in a dose-dependent manner. b The cells were treated with AMPK-specific siRNA (40 nM) or scrb siRNA for 48 h, then treated with Apelin 13 and I/R, followed by western blotting. c Effects of siAMPK on the cell viability. d PC12 cells were transfected with the empty vector pcDNA3 or vectors encoding HA-tagged wild-type GSK-3β (WT-GSK3β-HA). Upon transfection, PC12 cells were exposed to different treatments as indicated. e Nrf2 nuclear expression levels after transfected with GSK-3β. f Effects of GSK-3β-HA on the cell viability. The columns and error bars were represented as means ± SD. ^##P < 0.01 vs. the control group; **P < 0.01 vs. the I/R treatment group. ^&&P < 0.05 vs. the scrb control RNA group