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. 2019 Jan 31;17:9. doi: 10.1186/s12915-019-0629-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Three Cas12a nucleases generate slightly distinct mutation profiles that are unaffected by temperatures. OsDEP1 gene targeted by AsCas12a (a), FnCas12a (b), and LbCas12a (c). PAM is red, crRNA is blue. Error bars represent standard deviations of two biological replicates