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. 2019 Jan 11;17(1):50. doi: 10.3390/md17010050

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cytotoxic effects of EPS11 on Huh7.5 cells. (A) Purification and activity assay of EPS11. The profiles of the fractions in the gel filtration, which were collected and monitored for the cell proliferation determined at OD570 nm after 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and polysaccharide content determined at OD490 nm after the phenol-sulfuric acid assay. “Rcv” stands for relative cell viability. (B) Cytotoxic effects of EPS11 on Huh7.5 cells. Huh7.5 cells were seeded in a 96-well plate overnight, and treated with different concentrations of EPS11 for 24 hours and 48 hours. The cell viability was analyzed by MTT assay. Data were presented as means ± SD of three independent experiments (n = 3). *p < 0.05, ***p < 0.001.