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NTZ reverses the degradation of Smc5 induced by HBx. (A) HepG2 and (B) HepG2FlagHBx cells, which stably express Flag-HBx protein, were treated with NTZ or DMSO for 48 hours. The expression level of Smc5 protein was determined by Western blotting. Representative results from 3 independent experiments are shown. Summarized results of relative band intensities (n = 3) are shown below the panels. NS, not significant; *P = 5.4 × 10–3; **P = 1.1 × 10–5 (t test).
