Table 3.
List of mRNA Down-regulated by the TAM2 Subset (Myeloid Cells Scoring Gr1lowMHCIIlow) After Treatment With the CCL2 Inhibitor Subjected for Biocarta-Based Gene Group Analysis
| Gene symbol | Full name |
|---|---|
| Aire | Autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) |
| Atm | Ataxia telangiectasia mutated |
| Blnk | B-cell linker |
| C1ra | Complement component 1, r subcomponent A |
| C1s | Complement component 1s |
| C2 | Complement component 2 (within H-2S) |
| C4bp | Complement component 4 binding protein |
| C6 | Complement component 6 |
| C7 | Complement component 7 |
| C9 | Complement component 9 |
| Ccl22 | Chemokine (C-C motif) ligand 22 |
| Ccl26 | Chemokine (C-C motif) ligand 26 |
| Ccl8 | Chemokine (C-C motif) ligand 8 |
| Ccr6 | Chemokine (C-C motif) receptor 6 |
| Ccr7 | Chemokine (C-C motif) receptor 7 |
| Ccr9 | Chemokine (C-C motif) receptor 9 |
| Ccrl1 | C-C chemokine receptor type 11 |
| Cd163 | CD163 antigen |
| Cd19 | CD19 antigen |
| Cd1d1 | CD1d1 antigen |
| Cd209g | CD209g antigen |
| Cd22 | CD22 antigen |
| Cd247 | CD247 antigen |
| Cd28 | CD28 antigen |
| Cd34 | CD34 antigen |
| Cd3d | CD3 antigen, δ polypeptide |
| Cd4 | CD4 antigen |
| Cd40lg | CD40 ligand |
| Cd7 | CD7 antigen |
| Cd83 | CD83 antigen |
| Cd8a | CD8 antigen, α chain |
| Cd96 | CD96 antigen |
| Cdh5 | Cadherin 5 |
| Cfd | Complement factor D (adipsin) |
| Cish | Cytokine inducible SH2-containing protein |
| Ctla4 | Cytotoxic T-lymphocyte–associated protein 4 |
| Cxcl1 | Chemokine (C-X-C motif) ligand 1 |
| Cxcl12 | Chemokine (C-X-C motif) ligand 12 |
| Cxcl15 | Chemokine (C-X-C motif) ligand 15 |
| Defb1 | Defensin β 1 |
| Eomes | Eomesodermin |
| Fasl | Fas ligand (TNF superfamily, member 6) |
| Fcer1a | Fc receptor, IgE, high-affinity I, α polypeptide |
| Foxp3 | Forkhead box P3 |
| Gata3 | GATA binding protein 3 |
| Gp1bb | Glycoprotein Ib, β polypeptide |
| Gpr44 | Prostaglandin D2 receptor 2 |
| H2-Ob | Histocompatibility 2, O region β locus |
| H60a | Histocompatibility 60a |
| Hamp | Hepcidin antimicrobial peptide |
| Hcst | Hematopoietic cell signal transducer |
| Icam5 | Intercellular adhesion molecule 5, telencephalin |
| Icos | Inducible T-cell co-stimulator |
| Ifna1 | Interferon α 1 |
| Ikzf2 | IKAROS family zinc finger 2 |
| Ikzf3 | IKAROS family zinc finger 3 |
| Ikzf4 | IKAROS family zinc finger 4 |
| Il10 | Interleukin 10 |
| Il12a | Interleukin 12a |
| Il12rb1 | Interleukin 12 receptor, β 1 |
| Il12rb2 | Interleukin 12 receptor, β 2 |
| Il13 | Interleukin 13 |
| Il17a | Interleukin 17A |
| Il17b | Interleukin 17B |
| Il17f | Interleukin 17F |
| Il1a | Interleukin 1 α |
| Il1r1 | Interleukin 1 receptor, type I |
| Il1rl1 | Interleukin 1 receptor-like 1 |
| Il21 | Interleukin 21 |
| Il23a | Interleukin 23, α subunit p19 |
| Il28a | Interferon λ 2 |
| Il2ra | Interleukin 2 receptor, α chain |
| Il4 | Interleukin 4 |
| Il7r | Interleukin 7 receptor |
| Itga2b | Integrin α 2b |
| Itga6 | Integrin α 6 |
| Itgax | Integrin α X |
| Itln1 | Intelectin 1 (galactofuranose binding) |
| Kir3dl1 | Killer cell immunoglobulin-like receptor, 3 domains, long cytoplasmic tail, 1 |
| Kit | Kit oncogene |
| Klra21 | Killer cell lectin-like receptor subfamily A, member 21 |
| Klra5 | Killer cell lectin-like receptor, subfamily A, member 5 |
| Klrc1 | Killer cell lectin-like receptor subfamily C, member 1 |
| Klrc2 | Killer cell lectin-like receptor subfamily C, member 2 |
| Klrk1 | Killer cell lectin-like receptor subfamily K, member 1 |
| Lilra5 | Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5 |
| Ltb4r2 | Leukotriene B4 receptor 2 |
| Marco | Macrophage receptor with collagenous structure |
| Masp1 | Mannan-binding lectin serine peptidase 1 |
| Masp2 | Mannan-binding lectin serine peptidase 2 |
| Ms4a1 | Membrane-spanning 4-domains, subfamily A, member 1 |
| Nos2 | Nitric oxide synthase 2, inducible |
| Nox1 | NADPH oxidase 1 |
| Nox4 | NADPH oxidase 4 |
| Pdcd1 | Programmed cell death 1 |
| Pdgfb | Platelet-derived growth factor, B polypeptide |
| Pdgfrb | Platelet-derived growth factor receptor, β polypeptide |
| Pecam1 | Platelet/endothelial cell adhesion molecule 1 |
| Pigr | Polymeric immunoglobulin receptor |
| Pla2g2a | Phospholipase A2, group IIA (platelets, synovial fluid) |
| Pparg | Peroxisome proliferator activated receptor γ |
| Prf1 | Perforin 1 (pore-forming protein) |
| Prim1 | DNA primase, p49 subunit |
| Psmb11 | Proteasome (prosome, macropain) subunit, β type, 11 |
| Ptk2 | PTK2 protein tyrosine kinase 2 |
| Rag1 | Recombination activating gene 1 |
| Rorc | RAR-related orphan receptor γ |
| Runx3 | Runt-related transcription factor 3 |
| Sele | Selectin, endothelial cell |
| Sh2d1a | SH2 domain containing 1A |
| Slamf7 | SLAM family member 7 |
| Src | Rous sarcoma oncogene |
| Tbx21 | T-box 21 |
| Tcf7 | Transcription factor 7, T-cell specific |
| Tfrc | Transferrin receptor |
| Tgfb2 | Transforming growth factor, β |
| Thy1 | Thymus cell antigen 1, θ |
| Tigit | T-cell immunoreceptor with Ig and ITIM domains |
| Tlr3 | Toll-like receptor 3 |
| Tnfaip3 | Tumor necrosis factor, α-induced protein 3 |
| Tnfrsf13c | Tumor necrosis factor receptor superfamily, member 13c |
| Tnfrsf17 | Tumor necrosis factor receptor superfamily, member 17 |
| Tnfrsf9 | Tumor necrosis factor receptor superfamily, member 9 |
| Tnfsf10 | Tumor necrosis factor (ligand) superfamily, member 10 |
| Tnfsf11 | Tumor necrosis factor (ligand) superfamily, member 11 |
| Tnfsf14 | Tumor necrosis factor (ligand) superfamily, member 14 |
| Tnfsf15 | Tumor necrosis factor (ligand) superfamily, member 15 |
| Tnfsf8 | Tumor necrosis factor (ligand) superfamily, member 8 |
| Xcl1 | Chemokine (C motif) ligand 1 |
| Xcr1 | Chemokine (C motif) receptor 1 |
| Zap70 | Ζ-chain (TCR)–associated protein kinase |
NOTE. Mice at 14 days of age received DEN and twice-weekly injections of CCl4 for 16 weeks. In addition, mice were treated with CCL2i mNOXE36 or control inhibitor (revCCL2i) 3 times/wk subcutaneously. Peritumoral hepatic myeloid cells were isolated and subjected to quantitative analysis of mRNA expression (Nanostring immunology kit).
GATA, glutaminyl-tRNA synthase; ITIM, immunoreceptor tyrosine-based inhibition motif; NADPH, Nicotinamide adenine dinucleotide phosphate; PTK2, protein tyrosine kinase 2; RAR, retinoic acid receptor; SH2, Src Homology 2; SLAM, signaling lymphocytic activation molecule; TCR, T-cell receptor; TM, transmembrane; TNF, tumor necrosis factor.