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Berberine suppresses colorectal cancer viability by promoting apoptosis. (A) MTT analysis of cell viability after treatment with berberine at indicated concentrations and treatment duration. (B) Immunofluorescence analysis of Ki67 protein level in HT29 cells treated with berberine. (C) Apoptosis was detected by using FACS apoptosis assay in HT29 cells. (D) TUNEL assay was performed to verify the nuclear apoptosis in HT29 cells after treatment with berberine.
