Figure 4.

LncRNA CASC2 regulates Bcl-2 expression at post-transcriptional level. (A) FISH analysis of the subcellular location of lncRNA CASC2 with specific probe in colorectal cancer cells. (B) The expression level of lncRNA CASC2 in nuclear and cytoplasm of colorectal cancer cells. U1 (nuclear retained) and GAPDH (exported to cytoplasm) were used as controls. (C) Berberine treatment promoted the proportion of CASC2 expressed in cytoplasm. (D) RT-qPCR analysis of Bcl-2 mRNA level in cells silenced with lncRNA CASC2.