Figure 5.

LncRNA CASC2 sequesters AUF1 from binding to Bcl-2. (A) RNA immunoprecipitation (RIP) analysis by using anti-Bcl-2 antibody was performed to pull down CASC2, anti-IgG as negative control. (B) RIP assay by using anti-AUF1 antibody was done to pull down Bcl-2 and CASC2. (C) Western blot analysis was performed to verify the protein expression level of AUF1 and Bcl-2 after transfection of AUF1 overexpression vector. (D) RIP assay was performed to detect the influence of CASC2 knockdown on the interaction between AUF1 and Bcl-2, ** P<0.01.