Figure 1.

acDC Cytokine Cocktails Induce Equivalent APC Populations in CB and PB

PBMCs or CBMCs (2 × 10⁶ cells per well in 48-well plates) were cultured for 48 h with the indicated cytokine cocktails, namely, GM-CSF/IL-4, IL-1β, Flt3L, or no cytokines during 24 h followed by the addition of TNF-α, PGE2, IL-1β, and low-dose IL-7 for another 24 h. At the end of this 48-h culture, the phenotype of CD3⁻CD19⁻CD11c⁺ cells was assessed by flow cytometry using the indicated cell surface markers. (A) Representative staining obtained from one PBMC (white profiles) and one CBMC sample (gray profiles), as compared to isotype control (dotted line indicates the mean fluorescence intensity; MFI). (B) Cumulative data obtained from 3 CBMC and 3 PBMC donors, represented as relative MFI ± SD for each of the indicated markers, normalized to the MFI registered for PBMC samples in the absence of cytokines.