Figure 1.

Different nitrated ATI induce distinct TLR4 activation in HeLa TLR4 dual reporter cells. (A) HeLa TLR4 dual reporter cells were treated for 7 h with nitrated or unmodified ATI [7.5 μg/mL] or LPS [25 ng/mL] as a positive control. (B) Cells were pre-incubated for 2.5 h with TAK242 [0.36 μg/mL] or its solvent DMSO [4.4 μg/mL]. Then the cells were stimulated with nitrated or unmodified ATI [15 μg/mL], or LPS [25 ng/mL] for 7 h. The relative luciferase activity was calculated by dividing the Renilla luciferase (TLR4) signal by the Firefly luciferase (viability) signal. The resulting values were normalized to the value obtained for LPS- treated cells. Shown are the means ± SD of three independent experiments measured in triplicates using two independently nitrated probes. ^***P < 0.001, ^**P < 0.01.