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. 2019 Jan 21;9:3174. doi: 10.3389/fimmu.2018.03174

Figure 2.

Figure 2

Increased NF-κB p65 translocation into the cell nucleus in ATI and ATI TNM treated primary human macrophages. (A) Fluorescence microscopy images of macrophages treated for 2 h with LPS (positive control, 100 ng/mL), ATI or ATI TNM [12.5 μg/mL] and stained for NF-κB. In untreated conditions (medium) NF-κB resides mostly in the cytoplasm, whereas an increased translocation of NF-κB p65 into the nucleus can be observed for LPS, ATI, and ATI TNM treated cells (indicated by arrows). Brightness and contrast were adjusted to the same level for every image within the panel. Scale bar = 50 μm. (B) Quantitative evaluation of NF-κB translocation from the cell cytoplasm to the nucleus by Harmony high-content analysis software (Perkin Elmer). Shown are the means ± SEM from four independent experiments/donors, *P < 0.05, **P < 0.01. (C) Donor-dependent response to LPS (green), ATI (yellow), and ATI TNM (blue) treatment.