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. 2019 Jan 21;9:3174. doi: 10.3389/fimmu.2018.03174

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Copyright © 2019 Ziegler, Neumann, Liu, Fröhlich-Nowoisky, Cremer, Saloga, Reinmuth-Selzle, Pöschl, Schuppan, Bellinghausen and Lucas.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enhanced expression of maturation markers as well as pro-inflammatory cytokines by human immature DCs upon stimulation with ATI and ATI TNM. Immature DCs were pulsed with ATI or ATI TNM [15 μg/mL] on day 6. Forty-eight hours later cells were stained for expression of the indicated surface markers and analyzed by flow cytometry (A,B). Before, supernatants were taken for determination of IL-1β, IL-6, IL-8, and TNF-alpha by magnetic multiplex assay (C–F). Shown are the means ± SEM from ten independent experiments/donors, ^*P < 0.05, ^***P < 0.001.