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. 2019 Jan 22;9:3039. doi: 10.3389/fimmu.2018.03039

Figure 2.

Figure 2

Development and characterization of murine model of antibody-mediated heart allograft rejection. (A) BL/6 Tcrbd−/− recipients of BALB/c heart allografts were either unmodified (no cells group) or reconstituted the day after with 102, 103 (help-limited) or 5 × 105 (help-unlimited) CD4 T cells from TCR transgenic Rag1−/− TCR75 animals, which recognize I-Ab -restricted H-2Kd 54−68 peptide. (B) Development of anti-H-2Kd IgG antibody in reconstituted recipients (mean and S.E.M of n = 5 mice/group, *P < 0.001 for 5 × 105 vs. 103; p = 0.08 for 103 vs. 102 T cells, two-way ANOVA). (C) Reconstitution with limiting numbers of TCR75 CD4 T cells resulted in gradual allograft failure (MST = 50 days; n = 10), whereas grafts rejected acutely in help-unlimited recipients (MST = 9 days, P < 0.001, log-rank test; n = 10). Unmodified BL/6 Tcrbd−/− recipients that were not reconstituted (no cells, n = 6) did not reject the heart allograft.