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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: ACS Nano. 2018 Feb 22;12(3):2292–2310. doi: 10.1021/acsnano.7b07281

Figure 2.

Figure 2.

Deposition of pre- and post-incinerated organomodified montmorillonite nanoclay (300 μg/mouse) in day 1 postexposure animals. (a) H & E staining of terminal bronchiole (TB) and alveolar duct (AD) of exposed lung. 100× magnification. Green arrows indicate deposited particle associated with alveolar macrophages. (b) Polarized light microscopy of deposited particle in AD and alveoli of exposed lung. Images show particles (white) with polarized filter. 1000× magnification. CloisNa deposited in TB and AD regions as loose, stacked agglomerates or loosely dispersed in alveoli as small clusters and showed low polarization imaging potential (white arrows). FESEM/EDX elemental analysis confirmation of CloisNa within alveolar space (b, upper right). Clois30B was primarily found in TB and AD as large aggregates, with small aggregates in alveoli regions (black arrows). Both ICloisNa and I-Clois30B deposited primarily in TB and AD regions, while crystalline silica was found well-dispersed in alveoli.