Table 1.
Ion exchange chromatography procedure for Ru isolation and purification.
| Acid | Volume (ml) | Step |
|---|---|---|
| 1° cation column: 2–4 Biorad columns filled with 10 ml of pre-cleaned AG50WX8 (200–400 mesh) | ||
| Milli-Q water | 20 | Resin cleaning |
| 6M HCl | 10 + 10 + 10 + 10 | Resin cleaning |
| Milli-Q water | 20 | Resin cleaning |
| 0.15 M HCl | 10 | Backwash |
| 0.15 M HCl | 10 | Equilibration |
| 0.15 M HCl | 2.5 | Load |
| 0.15 M HCl | 1 + 1 +1 + 5 | Rinse HSE |
| 6 M HCl | 1 + 1 + 1 + 27 | Rinse matrix |
| 6 M HCl | 10 + 10 + 10 + 10 + 10 + 10 | Resin cleaning |
| Milli-Q water | 20 | Resin cleaning |
| 2° anion column: 1 Biorad column filled with 3.8 ml of pre-cleaned AG1X8 (200–400 mesh) | ||
| Milli-Q water | 10 | Resin cleaning |
| Concentrated HNO3 | 10 + 10 | Resin cleaning |
| Milli-Q water | 5 | Resin cleaning |
| Concentrated HCl | 10 | Resin cleaning |
| Milli-Q water | 5 | Resin cleaning |
| 1 M HCl | 3 | Backwash |
| 1 M HCl | 4 | Equilibration |
| 1 M HCl | 1 | Load |
| 0.5 M HCl | 2 | Matrix, Mo |
| 0.8 M HNO3 | 0.5 | Matrix, Mo |
| 0.8 M HNO3 | 5 | Zn, Cd, Mo |
| 8 M HNO3 | 10 | Ru, Re |
| Concentrated HNO3 | 4 | Ru, Re |
| Concentrated HNO3 | 10 | Pt, Ir |
| 11 M HCl | 2 | Pt, Ir, Ag |
| 11 M HCl | 8 | Pd |
| Concentrated HNO3 | 5 | Pd |