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. 2019 Feb 1;14(2):e0210402. doi: 10.1371/journal.pone.0210402

Fig 2. Characterization of gentamicin uptake, release, and antibacterial effects following loading onto the CaP coating.

Fig 2

A.) Uptake and release kinetics of gentamicin loading of CaP-coated TAN discs. 13 mm diameter TAN discs coated with CaP were immersed in 40 mg/ml gentamicin solution for varying periods (See figure legend: 1 s–filled circles, 1 min–filled squares, 5 min–filled triangles, 60 min–filled inverted triangles) before transfer into sterile PBS for 15 min (0–15), followed by further serial transfers into fresh PBS for defined periods (15–45 min; 45–90 min; 90–150 min). The horizontal line indicates the mean from 4–6 individual experiments. B.) Antibacterial efficacy of the elution solutions from part A. Zone of inhibition (ZOI) assays involving a gentamicin-sensitive S. aureus strain were performed to confirm the gentamicin released from the CaP coating retained antibacterial efficacy. Data shown are the mean of 3–6 independent experiments. *p<0.05; **p<0.01; ***p<0.001; n.s. = not significant.