Table 2.
Domain organization of the non-clip, multi-domain serine proteases and their homologs in the five model insects
| Name/feature | Domain structure | Dm | Ms | Ag | Am | Tc |
|---|---|---|---|---|---|---|
| Nudel | TM-3LDL-SP-2–4LDL-SPH-3/4LDL | Ndl/SP89 | SP50 | SP212 | SP20 | mSP19 |
| Tequila | S-2/15CBD-LDL-SR-LDL-SR-SP | Tequila/SP72 | - | SP213 | SP23* | mSP7* |
| Corin | TM-Fz-2LDL-SR-SP | Corin/SP75 | SP56 | SP214 | SP30 | mSP15 |
| /SEA | TM-SEA-Fz-2LDL-SPH | SPH198 | SPH145 | SPH216 | SPH56 | mSPH6 |
| ModSP | S-4/5LDL-Sushi-Wonton-SP | ModSP/SP53 | HP14a, HP14b | SP217 | SP49 | mSP3, 13 |
| /TSP | S-2TSP-SP | SP30 | SP55 | SP218 | SP38 | mSP2 |
| Masquerade | S-5/4Clip-SPH | Mas/cSPH79 | SPH53 | CLIPA15 | cSPH41, 39 | cSPH51 |
| /CUB | S-CUB-SP | SP120, SP80a-b | HP27 | SP201–6 | SP28, 34 | mSP9, 10 |
| /Gd | S-1/2Gd-SP | SP186/Gd, 55, 60, 212, SPH144, | SP45, 138, HP19 | SP207–10 | SP46 | mSP1, 4, 5, 11, 12 |
| GRAAL | TM-SEA-EGF-5LDL-SR-SPH | SP220 | SPH54 | mSPH16 | ||
| /SEA | TM-SEA-3LDL-Coil-SP | SP45 | ||||
| /SEA | TM-SEA-3LDL-SP-LDL | mSP8 | ||||
| /LamG | S-SP-Ig-2LamG | SP219 | ||||
| /Sushi | S-1/2/3Sushi-SP(H) | SP25, 112, SPH33 | ||||
| /2Sushi | S-2Sushi-SP-2Sushi-SP-2Sushi-SP | mSP14 |
CBD, type-2 chitin binding domain; CTL, C-type lectin domain; Coil, coiled coil region; CUB, a domain identified in complement 1r/s, uegf, and bmp1; EGF, a Ca2+-binding domain in epidermal growth factor; Gd, a conserved region first identified in DmSP186/Gd; Fz, frizzled domain; Ig, immunoglobulin domain; LamG, a domain in laminin γ-subunit; LDL, low-density lipoprotein receptor class A repeat; S, signal peptide; SEA, a domain identified in a sperm protein, enterokinase and agrin; SP, serine protease catalytic domain; SPH, non-catalytic serine protease homolog domain; SR, scavenger receptor Cys-rich domain; Sushi, Sushi domain, also known as CCP or SCR; TM, transmembrane region; TSP, type-1 repeat in thrombospondin-1; Wonton, a Sushi-like module first identified in M. sexta HP14 with six instead of four Cys residues.
AmSP23 and TcmSP7 are more complex in domain organization than AgSP213 or DmSP72/Tequila. In between the last CBD and the first LDL of Tequila, AmSP23 has SR-CTL-Kringle-LDL-Apple domains whereas TcmSP7 has only LDL-Apple domains.