Table 2.

Formulated and analyzed total AA content of experimental diets (%; as-fed basis)^{1, 2}

	ISS−				ISS+
	L1 formulated	L1 analyzed	L3 formulated	L3 analyzed	L1 formulated	L1 analyzed	L3 formulated	L3 analyzed
Isoleucine	0.52	0.54	0.87	0.85	0.44	0.45	0.75	0.75
Leucine	1.26	1.22	1.75	1.68	1.16	1.11	1.58	1.52
Lysine	0.89	0.94	1.40	1.38	0.77	0.79	1.21	1.26
Methionine	0.28	0.27	0.46	0.39	0.24	0.21	0.40	0.36
Cysteine	0.25	0.25	0.35	0.32	0.23	0.21	0.32	0.29
Phenylalanine	0.63	0.63	1.01	0.95	0.56	0.53	0.88	0.84
Threonine	0.50	0.51	0.78	0.78	0.44	0.43	0.69	0.68
Tryptophan	0.16	0.16	0.25	0.24	0.14	0.12	0.22	0.21
Valine	0.61	0.59	0.97	0.90	0.54	0.50	0.85	0.81

¹Thirty-nine gilts (initial BW 32 ± 2.1 kg) were subjected to one of two health states: immune system stimulated (ISS+; n = 24), and healthy control (ISS−; n = 15). Repeated intramuscular injection of E. coli lipopolysaccharide (25 and 35 µg/kg BW) was used to induce ISS. Diets were formulated to contain 70% (level 1, L1), 90% (L2), and 110% (L3) of daily standardized ileal digestible (SID) Thr requirements for each ISS group, which were estimated based on the potential of each group for PD according to the NRC Swine model (NRC, 2012). In all experimental diets, Thr was the first limiting among other AA. Diets L1 and L3 for each ISS group were prepared in single batches, and L2 diets were achieved by blending equal parts of L1 and L3.

²Analysis of the AA contents for the diets was performed at the Agricultural Experiment Station Chemical Laboratories at the University of Missouri-Columbia, MO.