. 2018 Nov 22;97(2):631–643. doi: 10.1093/jas/sky438

Table 2.

Comparison of microarray- and real-time RT-PCR (RT-PCR)-determined relative expression of prolactin and POMC/ACTH synthesis related genes in pituitary tissue of steers grazing endophyte-infected tall fescue and supplemented with 3 mg Se/d in vitamin–mineral mixes as either sodium selenite (ISe), SEL-PLEX (OSe), or a 1:1 mix of ISe and OSe (MIX)

Gene	Gene name	Microarray¹				RT-PCR²
		Treatment^3,4			P-value	Treatment^3,4			P-value
		ISe	MIX	OSe	P-value	ISe	MIX	OSe	P-value
Item
Prolactin synthesis-related genes
DRD2	Dopamine receptor D2	1.00^a	1.15^ab	1.27^b	0.027	1.13^a	1.64^ab	2.48^b	0.039
POU1F1	POU class 1 homeobox 1	1.00	1.06	1.05	0.530	1.02	1.11	0.87	0.277
PRL	Prolactin	1.00^a	1.12^ab	1.18^b	0.022	1.02^a	2.08^b	3.67^b	0.007
TRHR	Thyrotropin releasing hormone receptor	1.00^a	0.76^b	0.83^ab	0.045	1.00^a	0.71^b	0.81^ab	0.039
VIP	Vasoactive intestinal peptide	1.00	1.64	1.43	0.331	1.21	1.74	2.31	0.311
GAL	Galanin/GMAP prepropeptide	1.00	1.03	1.00	0.821	1.32	2.10	2.35	0.182
VEGFA	Vascular endothelial growth factor A	1.00^a	1.15^b	1.05^ab	0.093	1.01	1.18	1.03	0.250
TGFB1	Transforming growth factor beta 1	1.00^a	1.46^b	1.30^b	0.001	1.02^a	1.78^b	2.06^c	0.005
GHRHR	Growth hormone releasing hormone receptor	1.00	0.93	1.00	0.532	1.01	1.07	1.29	0.166
CSH2	Chorionic somatomammotropin hormone 2	1.00	1.09	0.97	0.173	1.10	0.74	0.87	0.279
PRLR	Prolactin receptor	1.00	1.13	1.23	0.141	NA	NA	NA	NA
L-PRLR	Prolactin receptor long isoform	NA	NA	NA	NA	1.01	0.95	1.17	0.376
S-PRLR	Prolactin receptor short isoform	NA	NA	NA	NA	1.01	1.01	1.09	0.761
POMC/ACTH/α-MSH synthesis related gene
POMC	Proopiomelanocortin	1.00^a	1.10^ab	1.23^b	0.045	1.14^a	1.91^a	4.06^b	0.002
PCSK1	Proprotein convertase subtilisin/kexin type 1	1.00^a	1.09^ab	1.44^b	0.059	1.03^a	1.48^ab	1.68^b	0.076
PCSK2	Proprotein convertase subtilisin/kexin type 2	1.00^a	1.13^ab	1.33^b	0.074	1.03^a	1.14^ab	1.41^b	0.048
CPE	Carboxypeptidase E	1.00^a	0.98^a	1.04^b	0.002	1.01^a	1.01^a	1.31^b	0.003
PAM	Peptidylglycine α-amidating monooxygenase	1.00^a	1.04^a	1.29^b	0.044	1.06^a	1.28^a	1.96^b	0.008

¹The abundance of gene transcripts is reported relative to the mean expression of the ISe treatment group and is expressed as fold-change of the untransformed intensity value.

²The abundance of gene transcripts is reported relative to the geometric mean expression of the reference genes.

³Values are least-squares means (n = 6 for ISe, n = 7 for OSe and MIX).

⁴Means within a row that lack a common letter differ (P < 0.05).