Serum titers against parainfluzenza3 virus (PI3; titer log 2) in cattle vaccinated against respiratory pathogens at: (i) weaning (day 0) and at feedlot entry (day 30; CON, n = 53), (ii) 15 d before weaning (day −15) and 15 d before feedlot entry (day 15; EARLY, n = 53), and (iii) 15 d after weaning (day 15) and 15 d after feedlot entry (day 45; DELAYED, n = 53). Vaccines administered were against Clostridium and Mannheimia haemolytica (2 mL subcutaneous injection of One Shot Ultra 7; Zoetis, Florham Park, NJ), bovine herpesvirus-1, bovine viral diarrhea viruses types 1 and 2, PI3, and bovine respiratory syncytial virus (2 mL subcutaneous injection of Bovi-Shield Gold 5; Zoetis). Calves not receiving vaccination were administered 4 mL of sterile saline. Panel A reports values relative to the day of the first vaccination (day 0) within each treatment, whereas asterisks in the X-axis indicate vaccination days. Panel B reports values during the experiment (days −15 to 75). Treatment × day interactions were detected (P ≤ 0.01). Within days, letters indicate (P ≤ 0.05): a = EARLY vs. CON, b = EARLY vs. DELAYED, c = CON vs. DELAYED.