Figure 1.

Expression of potential embryokines in equine endometrium, liver, and iTr cells. Total RNA was isolated from adult mare liver and pre- (Pr) and postovulation (PO) endometrium, and equine iTr cells, reverse transcribed and amplified with gene-specific primers for EGF, FGF2, HGF, IGF-1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Representative gel from a single mare endometrium sample is shown. Liver amplicons from two horses and iTr amplicons from two passages are shown in the lower panels. Amplicons were separated through Syber-safe agarose gels and imaged.