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Growth factor treatment of equine iTr cells differentially affects steroidogenic gene expression. Equine iTr cells were cultured for 36 h with 10 ng/mL of EGF, FGF2, HGF, or IGF-1. Total RNA was isolated, reverse transcribed, and analyzed by real-time PCR for Cyp11A, PTGS2, PTGES2, and PTGES3 mRNA abundance. Fold change calculated relative to CON cells treated with vehicle only. Mean and SEM of three replicate experiments shown. *Significance at P < 0.05.
