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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Brain Behav Immun. 2018 Oct 25;75:163–180. doi: 10.1016/j.bbi.2018.10.004

Fig. 1.

Fig. 1.

Vaccination with AβCore-S DNA vaccine at 6 months induces anti-Aβ IgM and IgG production in Ts65Dn and WT mice with high affinity to oligomers of murine Aβ. (A) Ts65Dn and WT mice were vaccinated at the age of 6 m with either AβCore-S or sham treatment for three times in a 14d interval. Total IgG, IgM and IgA titers as well as IgG subclasses were assessed every 3 months using indirect ELISA. (B) Antibodies found in the sera of AβCore-S-vaccinated mice effectively bind various Aβ42 oligomers (63,100,121.5–126 kDa), as assessed in western blot, compared to naïve serum (C) Total anti-Aβ IgG antibodies were significantly increased after immunization in both Ts65Dn (P < 0.0001) and WT (P < 0.0001) compared to baseline. Antibody levels remained high in the age of 12 m in Ts65Dn mice (P < 0.05) and 15 m (P < 0.001) in WT mice (D) Vaccinated Ts65Dn mice produce a higher IgM titer compare with their base line as well as with vaccinated WT mice. IgM levels remain high in this group until the age of 15 m. However, an age-dependent elevation in IgM levels was observed in both control groups. (E) IgG subclasses and IgA levels over time in vaccinated WT mice. IgG1 (P < 0.0001), IgG2b (P < 0.0001) and IgG3 (P < 0.001) were higher immediately after immunization and decreased to baseline by 9 m (F) IgG subclasses and IgA levels over time in vaccinated Ts65Dn mice. IgG1 (P < 0.0001) and IgG2b (P < 0.001) were higher immediately after immunization and decreased to baseline by 9 m (G) Anti-HBsAg IgG antibody levels increased in all groups immediately after immunization (P < 0.0001) and remained at high concentration until the age of 12 m for both Ts65Dn groups (P < 0.01) and 15 m for the WT/C, WT/V groups (P < 0.001, P < 0.05, respectively) (H) Anti-HBsAg IgM antibody levels increased in all groups immediately after immunization (P < 0.0001) and remained at high concentration until the age of 12 m for both transgenic groups (P < 0.01) and WT/C, WT/V groups (P < 0.0001, P < 0.001, respectively). Repeated-measures two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.