. Author manuscript; available in PMC: 2019 Feb 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Dec 13;1105:210–216. doi: 10.1016/j.jchromb.2018.12.009

Table 1.

Graduated elution liquid chromatography program for analysis of malondialdehyde-2,4-dinitrophenylhydrazine in exhaled breath condensate samples.

Time, min	Acetonitrile composition, %	Flow rate, mL/min	Valve position	Time, min	Acetonitrile composition (%)	Flow rate, mL/min	Valve position
0.0	3.0	0.5	To waste	10.7	100	0.3	To MS
4.5	27.0	0.5	To waste	11.0	100	0.3	To MS
5.0	29.6	0.4	To waste	11.7	100	0.5	To MS
6.2	36.0	0.37	To MS	12.9	100	0.5	To MS
7.2	37.6	0.35	To MS	13.0	51	0.5	To MS
7.5	38.0	0.3	To MS	13.1	3.0	0.4	To MS
10.5	42.7	0.3	To MS	14.0	3.0	0.4	To waste

Note: During chromatographic separation, a binary mobile phase composition consisting of Milli-Q® water with 0.05% formic acid (v/v) and acetonitrile without additives was used.