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BLI binding assays of ME07 towards recombinant human wtEGFR (a) and mutant EGFRvIII proteins (b). In these assays, the biosensors pre-coated with streptavidin interacted with biotinylated wtEGFR or EGFRvIII protein, followed by exposure to different concentrations of ME07 (0.97~1000 nM) for association and then to PBS for dissociation.
