Figure 1.

Physiological parameters in Irs2^LyzM−/−mice fed chow or HFD. (A) Energy expenditure (EE) in 8-week old control and Irs2^LyzM−/− males during both the day and night cycles (n = 22–26). (B, C) Percentage fat mass (B) and percentage lean mass (C) in 23 weeks old control and Irs2^LyzM−/− male mice on chow diet (n = 23–24). (D) Blood glucose levels in 8-week old control and Irs2^LyzM−/− female mice following an overnight fast (n = 20). (E) Serum insulin levels from 8-week old control and Irs2^LyzM−/− female mice following an overnight fast (n = 12–20). (F) Glucose tolerance in overnight fasted 8-week old female control and Irs2^LyzM−/− mice (n = 8–9). Inset shows means of area under the curve (AUC) for both genotypes. (G) Body weight of control and Irs2^LyzM−/- females fed either chow or high fat diet (HFD) from 8 weeks of age for 15 weeks (n = 8–11 for chow and n = 23 for HFD). (H, I) Percentage fat mass (H) and percentage lean mass (I) in 23-week old control and Irs2^LyzM−/− female mice fed HFD for 15 weeks (n = 23). (J) Serum leptin levels following an overnight fast in control and Irs2^LyzM−/− female fed HFD for 15 weeks (n = 10–12). (K) Energy expenditure (EE) values normalized to body weight in 23-week old control and Irs2^LyzM−/− females fed HFD for 15 weeks (n = 18–20). (L, M) Blood glucose (L) and serum insulin levels (M) in overnight fasted 16-week old control and Irs2^LyzM−/− females fed HFD for 8 weeks (n = 20–23). (N) Glucose tolerance in 6-hour fasted 16-week old control and Irs2^LyzM−/− female mice fed HFD for 8 weeks (n = 21). Inset shows means of area under the curve (AUC) for both genotypes. (O) Homeostatic model assessment for insulin resistance (HOMA-IR) calculated from fasted blood glucose and serum insulin levels from mice in L and M (n = 20). In all panels, data presented are mean ± SEM with Student's t test for all panels except F, G and O, where ANOVA with repeated measure was used. (*P < 0.05, **P < 0.01 and ***P < 0.001 relative to control mice).