Figure 4.

CX3CL1-Fc reduces monocyte cell adhesion to endothelial monolayers. (A) In vitro THP-1 adhesion assay under flow conditions. THP-1 cells pre-treated with CX3CL1-Fc are significantly less adherent to a HUVEC monolayer when under a shear stress of 1 dyne/cm² compared to VEH-treated controls. Representative images are shown (n = 3). (B–C) Static in vitro THP-1 adhesion assays. CX3CL1-Fc pre-treatment of THP-1 cells leads to reduced adhesion to a HUVEC monolayer under normal (B) and LPS-stimulated conditions (C). Representative images are shown (n = 3). (D–E) Static in vitro peripheral blood monocyte adhesion assays. Peripheral blood monocytes pre-treated with CX3CL1-Fc are less adherent to a HUVEC monolayer under normal (D) and LPS-stimulated (E) conditions. Representative images are shown (n = 3). Monocytes are shown in green and nuclei are labeled with DAPI. Scale bar = 100 μm. Data in both panels are presented as mean ± SD. *p < 0.05.