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. 2018 Nov 28;20:14–27. doi: 10.1016/j.molmet.2018.11.009

Figure 5.

Figure 5

Luciferase activity reflects UCP1 expression in primary adipocytes. A.In vitro bioluminescence imaging of primary brown and beige adipocytes in response to increasing concentrations of chronic Rosiglitazone (Rosi), acute isoproterenol (Iso, 100 nM), and acute retinoic acid (RA, 1000 nM) in the differentiation medium. B. Quantification of bioluminescence intensity, and corresponding detection of Luciferase, UCP1 and Actin protein levels, n = 3. Data were analyzed with one-way-ANOVA and compared to the control group, *P < 0.05, ****P < 0.0001 (Mean ± SD). C. Luciferase activity quantification and immunoblotting analysis of Rosiglitazone time-course effects during 7-day differentiation, which was divided into an early (first 4 days) and late (last 3 days) phases, n = 3. Data were analyzed with One-way-ANOVA, compared to the negative group (without Rosi), ****P < 0.001 (Mean ± SD).