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. 2018 Nov 28;20:14–27. doi: 10.1016/j.molmet.2018.11.009

Figure 6.

Figure 6

Cell-based imaging identifies a novel regulator of Ucp1 expression. A-B. Bioluminescence imaging of primary brown and beige adipocytes from KI mice in response to different concentrations of the SIKs inhibitor HG-9-91-01. C-D. Quantification of bioluminescence of primary brown and beige adipocytes in response to increasing concentrations of the SIKs inhibitor HG-9-91-01, n = 3. *P < 0.05, ***P < 0.001, ****P < 0.0001, compared to the control cells (Mean ± SD). E. Oxygen consumption rate (OCR) of immortalized brown adipocytes from 129S6Sv/EvTac mice. Cell cultures were chronically treated with 100 nM or 400 nM HG-9-91-01 during differentiation, n = 3. F. Quantification of basal, proton leak (oligomycin-insensitive OCR), UCP1-dependent respiration [(isoproterenol-stimulated OCR) – (oligomycin-resistant OCR)] and maximal respiration induced by FCCP, N = 3, n = 16. *P < 0.05, ****P < 0.0001, Two-way-ANOVA, (Mean ± SD).