Fig. 7.

Direct binding of Satb1 at the Bhlhe40 active promoter region. a ChIP-seq analyses of Satb1 binding and H3K27ac modification at the Bhlhe40 and Pdcd1 loci in in vitro polarized Th0 or eYFP⁺ Th17 cells. b Quantitative ChIP-PCR analysis for Satb1 binding to the Bhlhe40 promoter region. Chromatin from in vitro polarized Th0 or eYFP⁺ Th17 cells was immune-precipitated with anti-Satb1 antibody or control IgG. Percent of input before IP (1% gel input values) was quantified by real-time qPCR using primers specific for Bhlhe40 promoter region. The bar graph shows the mean ± s.d. (n = 3). The results are representative of more than two independent experiments. *P < 0.05; **P < 0.001 (two-tailed unpaired Student’s t-test)