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. 2019 Feb 1;10:556. doi: 10.1038/s41467-019-08442-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Extended information content in smart RESOLFT single recording. a The enhanced confocal, the enhanced negative image and the smart RESOLFT are all recorded in a smart pixel by a single scan of the sample. Each structure of the sample is illuminated according to probe type 2. The images are then combined to form the smart RESOLFT⁺⁺ image through a multi-image deconvolution (five iterations). Included for comparison is also the confocal image. b, c Zoom of two regions of interest, which highlight the increased spatial resolution of the smart RESOLFT and smart RESOLFT⁺⁺ images in comparison to the negative confocal, confocal and enhanced confocal images. Scale bars are 1 µm. RESOLFT REversible Saturable/Switchable Optical Linear Fluorescence Transitions