Fig. 4.

Smart RESOLFT imaging of hippocampal neurons. a, b Smart RESOLFT images of Synapsin1A-DronpaM159T, using probe type 2. The images were recorded 5.8 and 4.7 times faster than in conventional RESOLFT imaging with a reduced light dosage of 90% and 83% respectively. The insets show the decision maps with the percentage of skipped pixels (white, NO) and the fully illuminated pixels (black, YES). FOV of 40 by 40 µm² (a), and 30 by 15 µm² (b). I−III and V−IV region of interest (ROI) of images (a) and (b) respectively, showing single synaptic vesicles and small clusters. Scale bars are 10 µm (a), 5 µm (b), and 1 µm in ROI I−V. RESOLFT REversible Saturable/Switchable Optical Linear Fluorescence Transitions