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. 2019 Jan 17;116(5):1613–1620. doi: 10.1073/pnas.1817078116

Fig. 7.

Fig. 7.

The genomic ATG13-8SA mutant partially rescues the autophagy defect of the ptc2Δ ptc3Δ mutant strain. (A) GFP-Atg8 processing in strains that carry ATG13-8SA at the endogenous locus after 4-h rapamycin treatment. Samples were collected and blotted as previously described. (B) Quantification of GFP-Atg8 processing in WT, ptc2Δ ptc3Δ, ATG13-8SA, and ptc2Δ ptc3Δ ATG13-8SA strains from three independent experiments. Error bars represent SE (P = 0.04). (C) GFP-Atg8 localization in WT, ptc2Δ ptc3Δ, ATG13-8SA, and ptc2Δ ptc3Δ ATG13-8SA strains 4 h after rapamycin treatment.