Fig. 2.

Effect of Ani, Rapa, and DRB given into the vmPFC on the consolidation of the extinction of CFC with S. Animals with infusion cannulae implanted in the vmPFC were trained in CFC (three 2-s, 0.5-mA scramble foot shocks separated by 30-s intervals). After 24 h, the animals were submitted to an Ext Tr either A or with S. Immediately after the Ext Tr session, the animals were bilaterally infused intra-vmPFC with Veh, Ani (80 μg per side) (A), Rapa (5 μg per side) (B), or DRB (8 ng per side) (C). Twenty-four hours later, the animals underwent an Ext Test alone. When given into the vmPFC, Ani, Rapa, and DRB blocked the consolidation of the extinction of CFC. The figure shows the percentage of time spent freezing in the first 2 min of the Tr, in the first 3 min and the last 3 min of the Ext Tr, and in the Test. Data are expressed as mean ± SEM (n = 5–7 animals per group). **P < 0.01 and ***P < 0.001 vs. control groups in the retention Test, Bonferroni’s post hoc after two-way ANOVA. (Upper) Schematic of the behavioral protocol used.