Fig. 2.

Intestinal Arid1a deletion leads to decreased secretory cell lineages and increased apoptotic cells in the small intestines of mice. (A) IHC analysis for Lysozyme, Mmp7, Alcian blue, K_i67, and cleaved caspase 3 staining of the small intestines in control (Left) and Villin-Cre;Arid1a^f/f mice (Right) at 8–10 wk of age. (Scale bars, 100 µm.) (Inset magnification, 2.7×.) (B) Ratio of the number of Paneth cells to crypt cells in control (n = 5) and Villin-Cre; Arid1a^f/f mice (n = 4) at 8–10 wk of age. (C) Relative expression levels of Paneth cell markers in control and Villin-Cre;Arid1a^f/f mice, as determined by q-PCR using crypt RNA at 8 wk of age (n = 5). (D) Ratio of the number of goblet cells to crypt to villus cells in control and Villin-Cre;Arid1a^f/f mice at 8–10 wk of age (n = 3). (E) Ratio of the number of K_i67⁺ cells to crypt cells in control and Villin-Cre;Arid1a^f/f mice at 8–10 wk of age (n = 3). (F) Ratio of the number of crypts that contained at least one cleaved caspase 3⁺ cell to all crypt numbers in sections from control and Villin-Cre;Arid1a^f/f mice at 8–10 wk of age (n = 3). Quantitative data are presented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.