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. 2019 Jan 11;116(5):1704–1713. doi: 10.1073/pnas.1804858116

Fig. 7.

Fig. 7.

Sox9 overexpression restores ISCs in Arid1a mutant mice. (A) Macroscopic images of staining for LacZ and staining for Arid1a and LacZ in the small intestine in Lgr5CreERT2/+;RosalacZ/+;Arid1af/f;SOX9OE mice at 21 d after the last tamoxifen injection. [Scale bar for both panels, 100 µm.] (Inset magnification, 2.7×.) (B) The percentage of crypts containing at least one LacZ+ cell in the control Lgr5CreERT2/+;RosalacZ/+;Arid1a+/+, Lgr5CreERT2/+;RosalacZ/+;Arid1af/f, and Lgr5CreERT2/+;RosalacZ/+;Arid1af/f;SOX9OE mice at 8 wk of age (n = 3). (C) Staining for Musashi-1 in Villin-Cre;Arid1af/f;SOX9OE mice at 8 wk of age. (Scale bar, 100 µm.) (Inset magnification, 2.7×.) (D–F) Relative expression levels of intestinal stem cell markers (D), Wnt target genes (E), and Wnt agonist and receptor genes (F) in control, Villin-Cre;Arid1af/f, and Villin-Cre;Arid1af/f;SOX9OE intestines by q-PCR using crypt RNA at 8 wk of age (n = 5). (G) Relative expression levels of Wnt agonist genes in control, Villin-Cre;Arid1af/f, and Villin-Cre;Arid1af/f;SOX9OE intestines by q-PCR using whole tissue RNA at 8 wk of age (n = 3). Quantitative data are presented as means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.