Fig. 2.
None of the phages prey on V. cholerae in fresh water. Some 107 CFU of V. cholerae from an overnight culture were preadapted in autoclaved filter-sterilized fresh water overnight in the absence of an added carbon source. ICP1, ICP2, or ICP3 were added to a MOI of 0.01 to assess bacterial viability and phage replication over time. A and B show averages with standard errors. (A) Bacterial viability at 1, 2, 4, 7, and 24 h. (B) Phage adsorption after 15 min of static incubation at 24 °C. C and D show the medians with ranges of, at least, six biological replicates. The empty symbols represent data under the limit of detection. (C) The ratio of phages in the culture supernatant at 6 h relative to the number initially added. (D) The ratio of phages associated with V. cholerae at 6 h relative to the number initially added (*P < 0.05, **P < 0.005). PFU, plaque-forming units.