Fig. 4.

Vibrio cholerae is more susceptible to ICP1 and ICP2 in the presence of chitin. Some 10⁴ CFU of V. cholerae from an overnight culture were preadapted in a 1% chitin suspension in 0.7% Instant Ocean until exponential growth. ICP1, ICP2, or ICP3 were added to a MOI of 0.01 to assess bacterial viability and phage replication over time. Shown is the average with a SE of, at least, three biological replicates. (A) Bacterial viability at 16 h. (B) Phage replication at 16 h relative to the number initially added. (C) Some 10⁷ CFU of V. cholerae from an overnight culture were preadapted overnight for phage predation. ICP1, ICP2, or ICP3 were added to a MOI of 0.01. Bacterial viability at 1, 2, 4, 7, and 24 h in estuary conditions is shown. (D) The ratio of phages in the culture supernatant at 7 and 24 h relative to the number initially added (*P < 0.05, **P < 0.005, ****P < 0.0001). ns, not significant; PFU, plaque-forming units.