Fig. 1.
Inactivation of Abl1 in Scgb1a1+ lung epithelial cells protects mice from S. aureus-induced lung injury. (A) Timeline of experiment: Two weeks before nasal insufflation of 5 × 108 cfu S. aureus, both wild-type and Abl1fl/fl mice were treated with tamoxifen in CC10 (Scgb1a1)-CreERT2 mice. (B) Screenshot of Movie S1 of wild-type and knockout mice 24 h after nasal insufflation of S. aureus. (C) Two-minute tracing of mice performed with Adobe Premiere Pro showing a dramatic increase in mouse movement in knockout compared with wild-type mice. (D and E) Bronchioalveolar lavage showing reduced protein (D) and LDH (E) in knockout mice compared with wild-type mice. (F) H&E staining 3 d following nasal insufflation of S. aureus in wild-type and knockout mice showing increased protein and cell infiltrates in the airspace of wild-type mice compared with knockout mice. (A composite of images of mouse left lung sections using a 10× objective on the Zeiss AxioImager microscope stitched together with Zen software to recreate the entire left lung.) (G) Quantification of alveolar space infiltrates on H&E sections of wild-type vs. knockout mice normalized to a healthy, noninfected mouse. Graphs depict means and SEM of “n” mice, where n represents each individual animal used (i.e., n = 37 represents 37 individual mice). *P < 0.05, **P < 0.01, and ***P < 0.001.